93362
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93362
Sigma
Trizma® base
BioUltra, for molecular biology, ≥99.8% (T)
| Synonym: | 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol |
| CAS Number: | 77-86-1 |
| Linear Formula: | NH2C(CH2OH)3 |
| Molecular Weight: | 121.14 |
| Beilstein Registry Number: | 741883 |
| EC Number: | 201-064-4 |
| MDL number: | MFCD00004679 |
| PubChem Substance ID: | 24889854 |
Description
| Other Notes | Running buffer component in non-denaturing agarose gel electrophoresis1; In non-denaturing and urea-denaturing polyacrylamide gel electrophoresis2; DNA digest analysis with capillary electrophoresis3. |
| The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. | |
| Easily compare specifications for Trizma products with the Trizma specification table. | |
| Legal Information | Trizma is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| grade | for molecular biology |
| product line | BioUltra |
| assay | ≥99.8% (T) |
| total impurities | DNases, none detected |
| RNases, none detected | |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| ign. residue | ≤0.01% (as SO4) |
| loss | ≤0.5% loss on drying, 110 °C |
| pH | 10.5-12.0 (25 °C, 4 M in H2O) |
| useful pH range | 7 - 9 |
| pKa (25 °C) | 8.1 |
| bp | 219-220 °C/10 mmHg(lit.) |
| mp | 167-172 °C(lit.) |
| 168-172 °C | |
| solubility | H2O: 4 M at 20 °C, clear, colorless |
| anion traces | chloride (Cl-): ≤20 mg/kg |
| sulfate (SO42-): ≤5 mg/kg | |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| λ | 4 M in H2O |
| UV absorption | λ: 260 nm Amax: 0.10 |
| λ: 280 nm Amax: 0.08 |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Gloves |
| Hazard Codes | Xi |
| Risk Statements | 36 |
| Safety Statements | 26 |
| WGK Germany | 2 |
| RTECS | TY2900000 |
References
| Cited Reference | 1. R.C. Ogden, D.A. Adams Meth. Enzymol. 152, 78, (1987) |
| 2. R.C. Ogden, D.A. Adams Meth. Enzymol. 152, 65, (1987) | |
| 3. A. Paulus, D. Hüsken Electrophoresis 14, 27, (1993) Abstract | |
| reference | Molloy, M.P., et al. Electrophoresis 19, 837, (1998) |
| Santoni, V., et al. Electrophoresis 21, 1054, (2000) | |
| Merck | Merck 13,9842 |
| Beilstein | Beil. 4,IV,1903 |
| Aldrich MSDS 1, 1815:C / Corp MSDS 1 (2), 3532:A / FT-IR 1 (1), 347:C / FT-IR 2 (1), 542:D / IR-Spectra (3), 203:G / IR-Spectra (2), 183:E / IR-Spectra (2), 183:D / IR-Spectra (3), 203:H / NMR-Reference 2 (1), 304:A / RegBook 1 (1), 371:C / Sax 6, 2558 / Sigma FT-IR 1 (2), 60:D / Sigma FT-IR 1 (2), 61:C / Structure Index 1, 52:C:5 / Vapor Phase 3, 437:C |
